Hyun-Woo (Andrew) Park, Ph.D.

BIO114 General Botany with Lab
BIO143 Principles of Biology
BIO143L Principles of Biology Lab
BIO148 General Biology II with Lab
BIO205 Human Microbiology
BIO205L Human Microbiology Lab
BIO305 Microbiology with Lab
BIO380 Biology Seminar
BIO490 Research in Biology
BIO511 Techniques in Biomedical Sciences
BIO537 Bioethics
BIO590 Research in Biomedical Sciences
BIO592 Independent Reading

Molecular genetics of insect-pathogenic bacteria, Bacillus thuringiensis and Lysinibacillus sphaericus

Graduate-level Insect Molecular Genetics and Insect Pathology at Florida A&M University, Tallahassee, FL
Graduate-level Microbial Genetics at the University of California, Riverside, Riverside, CA
Undergraduate-level Forensic Entomology at Florida A&M University, Tallahassee, FL
Undergraduate-level Introduction to Biology at Community Christian College, Fontana, CA

REVIEWED: SCIENTIFIC JOURNAL PUBLICATIONS

Park, H.-W., H.-S. Kim, D.-W. Lee, Y.-M. Yu, B.-R. Jin and S.-K. Kang. 1995. Expression and synergistic effect of three types of crystal protein genes in Bacillus thuringiensis. Biochemical and Biophysical Research Communications 214, 602-607.

Roh, J.-Y., H.-W. Park, B.-R. Jin, H.-S. Kim, Y.-M. Yu and S.-K. Kang. 1996. Characterization of novel non-toxic Bacillus thuringiensis isolates from Korea. Letters in Applied Microbiology 23, 249-252.

Roh, J.-Y., H.-W. Park, Y.-H. Je, D.-W. Lee, B.-R. Jin, H.-W. Oh, S. S. Gill and S.-K. Kang. 1997. Expression of mosquitocidal crystal protein genes in noninsecticidal Bacillus thuringiensis subsp. israelensis. Letters in Applied Microbiology 24, 451-454.

Chang, J.-H., J.-Y. Roh, Y.-H. Je, H.-W. Park, B.-R. Jin, S.-D. Woo and S.-K. Kang. 1998. Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding δ-endotoxin Cry1E. Letters in Applied Microbiology 26, 387-390.

Park, H.-W., J.-Y. Roh, Y.-H. Je, B.-R. Jin, H.-W. Oh, H.-Y. Park and S.-K. Kang. 1998. Isolation of non-insecticidal Bacillus thuringiensis strain belonging to serotype H8a8b. Letters in Applied Microbiology 27, 62-66.

Park, H.-W., B. Ge, L. S. Bauer and B. A. Federici. 1998. Optimization of Cry3A yields in Bacillus thuringiensis by use of sporulation-dependent promoters in combination with the STAB-SD mRNA sequence. Applied and Environmental Microbiology 64, 3932-3938.

Chang, J.-H., Y.-H. Je, J.-Y. Roh, H.-W. Park, B.-R. Jin, D.-W. Lee, S.-H. Kim, Z.-W. Yang and S.-K. Kang. 1999. Isolation and characterization of a strain of Bacillus thuringiensis serovar kenyae encoding only delta-endotoxin Cry1E. Applied Entomology and Zoology 34, 379-382.

Park, H.-W., D. K. Bideshi, J. J. Johnson and B. A. Federici. 1999. Differential enhancement of Cry2A versus Cry11A yields in Bacillus thuringiensis by use of the cry3A STAB mRNA sequence. FEMS Microbiology Letters 181, 319-327.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2000. Molecular genetic manipulation of truncated Cry1C protein synthesis in Bacillus thuringiensis to improve stability and yield. Applied and Environmental Microbiology 66, 4449-4455.

Park, H.-W. and B. A. Federici. 2000. Domain I plays an important role in the crystallization of Cry3A in Bacillus thuringiensis. Molecular Biotechnology 16, 97-107.

Park, H.-W., A. Delécluse and B. A. Federici. 2001. Construction and characterization of a recombinant Bacillus thuringiensis subsp. israelensis strain that produces Cry11B. Journal of Invertebrate Pathology 78, 37-44.

Je, Y.-H., B.-R. Jin, H.-W. Park, J.-Y. Roh, J.-H. Chang, S.-J. Seo, J. A. Olszewski, D. R. O’Reilly and S.-K. Kang. 2003. Baculovirus expression vectors that incorporate the foreign protein into viral occlusion bodies. BioTechniques 34, 81-87.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2003. Recombinant strain of Bacillus thuringiensis producing Cyt1A, Cry11B and the Bacillus sphaericus binary toxin. Applied and Environmental Microbiology 69, 1331-1334.

Park, H.-W. and B. A. Federici. 2004. Effect of specific mutations in helix alpha-7 of Domain I on the stability and crystallization of Cry3A in Bacillus thuringiensis. Molecular Biotechnology 27, 89-100.

Wirth, M. C., H.-W. Park, W. E. Walton and B. A. Federici. 2005. Cyt1A of Bacillus thuringiensis delays evolution of resistance to Cry11A in the mosquito, Culex quinquefasciatus. Applied and Environmental Microbiology 71, 185-189.

Hughes, P. A., M. M. Stevens, H.-W. Park, B. A. Federici, E. S. Dennis and R. Akhurst. 2005. Response of larval Chironomus tepperi (Diptera: Chironomidae) to individual Bacillus thuringiensis var. israelensis toxins and toxin mixtures. Journal of Invertebrate Pathology 88, 34-39.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2005. Synthesis of additional endotoxins in Bacillus thuringiensis subsp. morrisoni PG-14 and Bacillus thuringiensis subsp. jegathesan significantly improves their mosquitocidal efficacy. Journal of Medical Entomology 42, 337-341.

Park, H.-W., D. K. Bideshi, M. C. Wirth, J. J. Johnson, W. E. Walton and B. A. Federici. 2005. Recombinant larvicidal bacteria with markedly improved efficacy against Culex vectors of West Nile Virus. The American Journal of Tropical Medicine and Hygiene 72, 732-738.

Tang, M., D. K. Bideshi, H.-W. Park and B. A. Federici. 2006. A mini-replicon from pBtoxis of Bacillus thuringiensis subsp. israelensis. Applied and Environmental Microbiology 72, 6948-6954.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2007. The 20-kDa protein of Bacillus thuringiensis subsp. israelensis enhances Bacillus sphaericus 2362 Bin toxin synthesis. Current Microbiology 55, 119-124.

Tang, M., D. K. Bideshi, H.-W. Park and B. A. Federici. 2007. Iteron-binding ORF157 and FtsZ-like ORF156 proteins encoded by pBtoxis play a role in its replication in Bacillus thuringiensis subsp. israelensis. Journal of Bacteriology 189, 8053-8058.

Park, H.-W., C. M. Mangum, H. Zhong and S. R. Hayes. 2007. Isolation of Bacillus sphaericus with improved efficacy against Culex quinquefasciatus. Journal of the American Mosquito Control Association 23, 478-480.

Park, H.-W., S. R. Hayes and C. M. Mangum. 2008. Distribution of mosquitocidal Bacillus thuringiensis and Bacillus sphaericus from sediment samples in Florida. Journal of Asia-Pacific Entomology 11, 217-220.

Park, H.-W., S. R. Hayes, G. M. Stout, G. Day-Hall, M. D. Latham and J. P. Hunter. 2009. Identification of two mosquitocidal Bacillus cereus strains showing different host ranges. Journal of Invertebrate Pathology 100, 54-56.

Park, H.-W., M. Tang, Y. Sakano and B. A. Federici. 2009. A 1.1-kilobase region downstream of the bin operon in Bacillus sphaericus strain 2362 decreases Bin yield and crystal size in strain 2297. Applied and Environmental Microbiology 75, 878-881.

Hayes, S. R., M. Hudon and H.-W. Park. 2011. Isolation of novel Bacillus species showing high mosquitocidal activity against several mosquito species. Journal of Invertebrate Pathology 107, 79-81.

Park, H.-W., J. A. Devera, B. A. Prins and D. K. Bideshi. 2011. The dual-activity insecticidal protein, Cry2Aa, does not enhance the mosquitocidal activity of Bacillus thuringiensis subsp. israelensis. Journal of Asia-Pacific Entomology 14, 429-431.

Barboza-Corona, J. E., H.-W. Park, D. K. Bideshi and B. A. Federici. 2012. The 60-kDa protein encoded by orf2 in the cry19A operon of Bacillus thuringiensis subsp. jegathesan functions as a C-terminal crystallization domain. Applied and Environmental Microbiology 78, 2005-2012.

Park, H.-W., B. C. Pino, S. Kozervanich-Chong, E. A. Hafkenscheid, R. M. Oliverio, B. A. Federici and D. K. Bideshi. 2013. Cyt1Aa from Bacillus thuringiensis subsp. israelensis enhances mosquitocidal activity of B. thuringiensis subsp. kurstaki HD-1 against Aedes aegypti but not Culex quinquefasciatus. Journal of Microbiology and Biotechnology 23, 88-91.

Sun, Y., Q. Zhao, L. Xia, X. Ding, Q. Hu, B. A. Federici and H.-W. Park. 2013. Identification and characterization of three previously undescribed crystal proteins from Bacillus thuringiensis subsp. jegathesan. Applied and Environmental Microbiology 79, 3364-3370.

Bideshi, D. K., G. Waldrop, M. T. Fernandez-Luna, M. Diaz-Mendoza, M. C. Wirth, J. J. Johnson, H.-W. Park and B. A. Federici. 2013. Intermolecular interaction between Cry2Aa and Cyt1Aa and its effect on larvicidal activity against Culex quinquefasciatus. Journal of Microbiology and Biotechnology 23, 1107-1115.

Sun, Y., Q. Zhao, D. Zheng, X. Ding, J. Wang, Q. Hu, Z. Yuan, H.-W. Park and L. Xia. 2014. Construction and characterization of the interdomain chimeras using Cry11Aa and Cry11Ba from Bacillus thuringiensis and identification of a possible novel toxic chimera. Biotechnology Letters 36, 105-111.

Sawaya, M. R., D. Cascio, M. Gingery, J. Rodriguez, L. Goldschmidt, J.-P. Colletier, M. M. Messerschmidt, S. Boutet, J. E. Koglin, G. J. Williams, A. S. Brewster, K. Nass, J. Hattne, S. Botha, R. B. Doak, R. L. Shoeman, D. P. DePonte, H.-W. Park, B. A. Federici, N. K. Sauter, I. Schlichting and D. S. Eisenberg. 2014. Protein crystal structure obtained at 2.9 Å resolution from injecting bacterial cells into an X-ray free-electron laser beam. Proceedings of the National Academy of Sciences, U.S.A. 111, 12769-12774.

Park, H.-W., R. H. Hice and B. A. Federici. 2016. Effect of promoters and plasmid copy number on Cyt1A synthesis and crystal assembly in Bacillus thuringiensis. Current Microbiology 72, 33-40.

Colletier, J.-P., M. R. Sawaya, M. Gingery, J. A. Rodriguez, D. Cascio, A. S. Brewster, T. Michels-Clark, R. H. Hice, N. Coquelle, S. Boutet, G. J. Williams, M. Messerschmidt, D. P. DePonte, R. G. Sierra, H. Laksmono, J. E. Koglin, M. S. Hunter, H.-W. Park, M. Uervirojnangkoorn, D. K. Bideshi, A. T. Brunger, B. A. Federici, N. K. Sauter and D. S. Eisenberg. 2016. Mosquito larvicide BinAB revealed by de novo phasing with an X-ray laser. Nature 539, 43-47.

Sakano, Y., H.-W. Park, B. Ge, D. K. Bideshi and B. A. Federici. 2017. Contributions of 5’-UTR and 3’-UTR cis elements to Cyt1Aa synthesis in Bacillus thuringeinsis subsp. israelensis. Journal of Invertebrate Pathology 149, 66-75.

Bideshi, D. K., H.-W. Park, R. H. Hice, M. C. Wirth and B. A. Federici. 2017. Highly effective broad spectrum chimeric larvicide that targets vector mosquitoes using a lipophilic protein. Scientific Reports 7, 11282.

Tetreau, G., A.-S. Banneville, E. A. Andreeva, A. S. Brewster, M. S. Hunter, R. G. Sierra, J.-M. Teulon, I. D. Young, N. Burke, T. A. Grünewald, J. Beaudouin, I. Snigireva, M. T. Fernandez-Luna, A. Burt, H.-W. Park, L. Signor, J. A. Bafna, R. Sadir, D. Fenel, E. Boeri-Erba, M. Bacia, N. Zala, F. Laporte, L. Després, M. Weik, S. Boutet, M. Rosenthal, N. Coquelle, M. Burghammer, D. Cascio, M. R. Sawaya, M. Winterhalter, E. Gratton, I. Gutsche, B. Federici, J.-L. Pellequer, N. K. Sauter, J.-P. Colletier. 2020. Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade. Nature Communications 11, 1153.

Tetreau, G., M. R. Sawaya, E. D. Zitter, E. A. Andreeva, A.-S. Banneville, N. Schibrosky, N. Coquelle, A. S. Brewster, M. L. Grünbein, G. N. Kovacs, M. S. Hunter, M. Kloos, R. G. Sierra, G. Schiro, P. Qiao, M. Stricker, D. Bideshi, I. D. Young, N. Zala, S. Engiberge, A. Gorel, L. Signor, J.-M. Teulon, M. Hilpert, L. Foucar, J. Bielecki, R. de Wijn, T. Sato, H. Kirkwood, R. Bean, R. Letrun, A. Batyuk, I. Snigireva, D. Fenel, R. Schubert, E. J. Canfield, M. M. Alba, F. Laporte, L. Després, M. Bacia, A. Roux, C. Chapelle, F. Riobé, O. Maury, W. L. Ling, S. Boutet, A. Mancuso, I. Gutsche, E. Girard, T. R. M. Barends, J.-L. Pellequer, H.-W. Park, A. D. Laganowsky, J. Rodriguez, M. Burghammer, R. L. Shoeman, R. B. Doak, M. Weil, N. K. Sauter, B. Federici, D. Cascio, I. Schlichting and J.-P. Colletier. 2022. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals. Nature Communications 13, 4376.

Williamson, L. J., M. Galchenkova, H. L. Best, R. Bean, A. Munke, S. Awel, G. Pena, J. Knoska, R. Schubert, K. Dörner, H.-W. Park, D. K. Bideshi, A. Henkel, V. Kremling, B. Klopprogge, E. Lloyd-Evans, M. T. Young, J. Valerio, M. Kloos, M. Sikorski, G. Mills, J. Bielecki, H. Kirkwood, C. Kim, R. de Wijn, K. Lorenzen, L. Xavier, A. Rahmani, L. Gelisio, O. Yefanov, A. P. Mancuso, B. A. Federici, H. N. Chapman, N. Crickmore, P. J. Rizkallah, C. Berry and D. Oberthür. 2023. Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX. Proceedings of the National Academy of Sciences, U.S.A. 120, e220341120.

Rudd, S. R., L. S. Miranda, H. R. Curtis, Y. Bigot, M. M. Diaz-Mendoza, R. Hice, V. Nizet, H.-W. Park, G. Blaha, B. A. Federici and D. K. Bideshi. 2023. The parasporal body of Bacillus thuringiensis subsp. israelensis: A unique phage capsid-associated prokaryotic insecticidal organelle. Biology 12, 1421.

INVITED CHAPTERS AND REVIEW ARTICLES:

Federici, B. A., H.-W. Park, D. K. Bideshi and B. Ge. 2000. Chapter 7.2 Genetic engineering of bacterial insecticides for improved efficacy against medically important diptera. In “Entomopathogenic Bacteria: from Laboratory to Field Application.” (J. F. Charles, A. Delécluse and C. Niesen-LeRoux eds.), pp. 461-484. Kluwer Academic Publishers, Amsterdam.

Park, H.-W. and B. A. Federici. 2000. Genetic factors affecting insecticidal crystal protein synthesis in Bacillus thuringiensis. International Journal of Industrial Entomology 1, 1-7.

Federici, B. A., H.-W. Park, D. K. Bideshi, M. C. Wirth and J. J. Johnson. 2003. Recombinant bacteria for mosquito control. Journal of Experimental Biology 206, 3877-3885.

Federici, B. A., H.-W. Park and Y. Sakano. 2006. Insecticidal protein crystals of Bacillus thuringiensis. In Microbiology Monographs, Volume 1, “Inclusions in Prokaryotes” (J. M. Shively ed.), pp. 195-236. Springer-Verlag, Heidelberg.

Park, H.-W., B. A. Federici and Y. Sakano. 2006. Inclusion proteins from other insecticidal bacteria. In Microbiology Monographs, Volume 1, “Inclusions in Prokaryotes” (J. M. Shively ed.), pp. 321-330. Springer-Verlag, Heidelberg.

Federici, B. A., H.-W. Park, D. K. Bideshi, M. C. Wirth, J. J. Johnson, Y. Sakano and M. Tang. 2007. Developing recombinant bacteria for control of mosquito larvae. In “Biorational Control of Mosquitoes.” (T. G. Floore ed.), pp. 164-175. American Mosquito Control Association, Mount Laurel.

Park, H.-W. 2008. Current status of larvicidal bacteria for mosquito control: A review. Technical Bulletin of the Florida Mosquito Control Association 8, 31-35.

Park, H.-W. and B. A. Federici. 2009. Chapter 12. Genetic engineering of bacteria to improve efficacy using the insecticidal proteins of Bacillus species. In “Insect Pathogens – Molecular Approaches and Techniques.” (S. P. Stock, J. Vandenberg, I. Glazer and N. Boemare eds.), pp. 275-305. CABI Publishing, Cambridge.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2010. Properties and applied use of the mosquitocidal bacterium, Bacillus sphaericus. Journal of Asia-Pacific Entomology 13, 159-168.

Federici, B. A., H.-W. Park and D. K. Bideshi. 2010. Overview of the Basic biology of Bacillus thuringiensis with emphasis on genetic engineering of bacterial larvicides for mosquito control. In “Entomopathogenic Bacterial Toxins as Biological Control Agents.” (A. Zaritsky and E. Ben-Dov eds.). Supplementary Issue, The Open Toxinology Journal 3, 154-171.

Barboza-Corona, J. E., D. K. Bideshi, H.-W. Park and R. Salcedo-Hernández. 2023. Editorial: Novel research on metabolites secreted by Gram-positive bacteria. Frontiers in Microbiology 14:1237790.

PROCEEDINGS OF SYMPOSIA OR TECHNICAL MEETINGS:

Federici, B. A., H.-W. Park and D. K. Bideshi. 2001. Improving the efficacy of Bacillus thuringiensis insecticides: From molecular biology to practical applications. In “Proceedings of the Centennial Symposium Commemorating Ishiwata's Discovery of Bacillus thuringiensis.” (M. Ohba, O. Nakamura, E. Mizuki and T. Akao eds.), pp. 15-20. Kurume, Japan.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson and M. C. Wirth. 2001. Synergistic interactions that enhance the pathogenicity of Bacillus thuringiensis. In “Proceedings of the 4th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Environmental Impact.” pp. 25-31. Canberra, Australia.

Park, H.-W. 2007. Molecular genetic strategies to enhance efficacy of entomopathogenic bacteria. In “Proceedings of the Korea Conference on Innovative Science and Technology 2007. Molecular Host – Parasite Interactions: New Horizon on Interface Biology.” pp. 159-168. Jeju, Korea.

Park, H.-W., S. R. Hayes and C. M. Mangum. 2007. Biological control of mosquitoes: Microbiology, molecular genetics and biophysics. In “Proceedings of the CESTA 2007 Research Forum.” pp. 37-38. Florida A & M University, Tallahassee, Florida.

Park, H.-W. 2008. Entomopathogenic bacteria for vector control: Molecular genetic exploration and novel toxin discovery. In “Proceedings of the 1st China – Korea Joint Symposium on Insect Biotechnology.” p. 23. Wuhan, China.

Park, H.-W. 2009. Discovery of novel mosquitocidal bacteria from mosquito larval habitats. In “Proceedings of the 1st International Symposium on Insect Biotechnology & 2nd Korea – China Joint Symposium on Insect Biotechnology.” p. 26. Busan, Korea.

JOURNAL SPECIAL TOPICS EDITOR:

Bideshi, D. K., H.-W. Park, J. E. Barbosa-Corona and R. Salcedo-Hernández. 2019. Frontiers in Microbiology, Novel Research on Metabolites Secreted by Gram-Positive Bacteria.

PATENTS:

Kang, S.-K., H.-S. Kim, H.-W. Park, B.-R. Jin, S.-D. Woo, Y.-H. Je, D.-W. Lee, J.-Y. Roh, J.-H. Chang and Y.-M. Yu. 1998. Insecticidal protein of Bacillus thuringiensis NT0423 and microbial insecticide using it. Korea Patent No. 98-3455.

Kang, S.-K., Y.-H. Je, B.-R. Jin, H.-W. Park, J.-Y. Roh and J.-H. Chang. 1998. Recombinant baculovirus, process for preparing thereof and microbial insecticide containing it. Korea Patent No. 98-11914.

Kang, S.-K., Y.-H. Je, B.-R. Jin, H.-W. Park, J.-Y. Roh and J.-H. Chang. 1998. Recombinant baculovirus, construction method thereof and insect pesticidal composition containing the same. U.S. Patent No. 6,338,846.

Federici, B. A., D. K. Bideshi, H.-W. Park and M. C. Wirth. 2004. Insecticidal bacteria, and methods for making and using them. U.S. Patent No. 6,720,167.

Park, H.-W., C. M. Mangum and H. Zhong. 2007. A novel mosquitocidal bacterium and its toxin. U.S. Patent Application No. 11/770,203.

Park, H.-W., S. R. Hayes, G. M. Stout and M. D. Latham. 2008. A novel Bacillus cereus mosquitocidal bacterium mainly active against Ochlerotatus taeniorhynchus and its toxin. U.S. Patent Application No. 122,838.

Park, H.-W., S. R. Hayes, G. Day Hall and J. P. Hunter. 2008. A novel Bacillus cereus mosquitocidal bacterium mainly active against Culex quinquefasciatus and its toxin. U.S. Patent Application No. 122,839.

Park, H.-W., S. R. Hayes and M. Hudon. 2010. New strain of highly mosquitocidal Bacillus. U.S. Patent Application No. 12/754,259.

Park, H.-W., S. R. Hayes and M. Hudon. 2010. New strain of highly mosquitocidal Bacillus. U.S. Patent Application No. 12/754,322.

Federici, B. A., D. K. Bideshi and H.-W. Park. 2015. Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications. UC Disclosure Ref. No. 2015-442-1

Federici, B. A., D. K. Bideshi, H.-W. Park, R. H. Hice and T. Su. 2016. Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications. U.S. Provisional Patent Application Serial No. 62/300,489

Federici, B. A., D. K. Bideshi, H.-W. Park, R. H. Hice and T. Su. 2017. Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications. U.S. Non-Provisional Patent Application Serial No. 15/646,062

Saleem, F., H.-W. Park, S. Naz and A. R. Shakoori. 2018. Process for recombinant protein expression augmentation in Bacillus expression system. U.S. Patent No. 9,951,343

Federici, B. A., D. K. Bideshi, H.-W. Park, R. H. Hice and T. Su. 2019. Use of Cyt1Aa as broad spectrum targeting domain for insecticidal proteins with basic research and biotechnology applications. U.S. Patent No. 10472397

CONTRIBUTED PAPER PRESENTATIONS:

Park, H.-W., B. Ge, L. S. Bauer and B. A. Federici. 1998. Very high levels of Cry3A synthesis are obtained in Bacillus thuringiensis using dual sporulation-dependent promoters in combination with the STAB-SD mRNA sequence. 31st Annual Meeting of the Society for Invertebrate Pathology, VIIth International Colloquium on Invertebrate Pathology and Microbial Control, and IVth International Conference on Bacillus thuringiensis. Sapporo, Japan.

Roh, J.-Y., H.-W. Park, Y.-H. Je, J.-H. Chang, B.-R. Jin and S.-K. Kang. 1998. Isolation of a noninsecticidal Bacillus thuringiensis strain belonging to subsp. morrisoni (H8a8b). 31st Annual Meeting of the Society for Invertebrate Pathology, VIIth International Colloquium on Invertebrate Pathology and Microbial Control, and IVth International Conference on Bacillus thuringiensis. Sapporo, Japan.

Chang, J.-H., J.-Y. Roh, Y.-H. Je, H.-W. Park, B.-R. Jin, Z.-W. Yang and S.-K. Kang. 1998. Isolation of Spodoptera-specific Bacillus thuringiensis strains in Korea. 2nd International Symposium on Biopesticides. Wuhan, China.

Ge, B., H.-W. Park and B. A. Federici. 1998. Regulation of crystal protein synthesis and crystallization in Bacillus thuringiensis. 2nd International Symposium on Biopesticides. Wuhan, China.

Park, H.-W. and B. A. Federici. 1999. Domain I plays a key role in Cry3A crystallization. 32nd Annual Meeting of the Society for Invertebrate Pathology. Irvine, California.

Park, H.-W. and B. A. Federici. 2000. Structural role for Domain I helix alpha-7 in Cry3A crystallization in vivo in Bacillus thuringiensis. 33rd Annual Meeting of the Society for Invertebrate Pathology and Vth International Conference on Bacillus thuringiensis. Guanajuato, Mexico.

Federici, B. A., M. C. Wirth, D. K. Bideshi, H.-W. Park and W. E. Walton. 2000. Role of Cyt1A in managing resistance to mosquitocidal proteins of Bacillus thuringiensis and Bacillus sphaericus. 33rd Annual Meeting of the Society for Invertebrate Pathology and Vth International Conference on Bacillus thuringiensis. Guanajuato, Mexico.

Park, H.-W., A. Delecluse and B. A. Federici. 2000. Properties of a recombinant Bacillus thuringiensis subsp. israelensis IPS-82 that produces Cry11B. 33rd Annual Meeting of the Society for Invertebrate Pathology and Vth International Conference on Bacillus thuringiensis. Guanajuato, Mexico.

Federici, B. A., D. K. Bideshi, H.-W. Park, M. C. Wirth, W. E. Walton and J. J. Johnson. 2000. Markedly improved bacterial insecticides for vectors by combining Bti and Bs proteins. 33rd Annual Meeting of the Society for Invertebrate Pathology and Vth International Conference on Bacillus thuringiensis. Guanajuato, Mexico.

Federici, B. A., D. K. Bideshi, H.-W. Park, M. C. Wirth, W. E. Walton and J. J. Johnson. 2001. Genetically engineered bacteria containing Bti and Bs proteins for improved control of nuisance and vector mosquito larvae. 69th Annual Conference of the Mosquito and Vector Control Association of California. Napa, California.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2001. Construction and characterization of a Bacillus thuringiensis strain producing Cry11B, Cyt1A and Bacillus sphaericus 2362 binary toxin. 34th Annual Meeting of the Society for Invertebrate Pathology. Noordwijkerhout, The Netherlands.

Federici, B. A., D. K. Bideshi, H.-W. Park, M. C. Wirth, W. E. Walton and J. J. Johnson. 2001. Markedly improved recombinant Bacillus thuringiensis subsp. israelensis that synthesizes a high level of the Bacillus sphaericus binary toxin. 34th Annual Meeting of the Society for Invertebrate Pathology. Noordwijkerhout, The Netherlands.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson, M. C. Wirth and W. E. Walton. 2001. New recombinant bacterial larvicides for mosquito control. 2001 Entomological Society of America Annual Meeting. San Diego, California.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson, M. C. Wirth and W. E. Walton. 2002. New recombinant bacteria for mosquito control. 70th Annual Conference of the Mosquito and Vector Control Association of California. Fish Camp, California.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2002. Addition of the Bacillus sphaericus binary toxin to Bacillus thuringiensis subsp. morrisoni (PG-14) and Bacillus thuringiensis subsp. jegathesan markedly improves their toxicity. 35th Annual Meeting of the Society for Invertebrate Pathology, VIIIth International Colloquium on Invertebrate Pathology and Microbial Control, and VIth International Conference on Bacillus thuringiensis. Fos do Iguassu, Brazil.

Park, H.-W., B. A. Federici, D. K. Bideshi, M. C. Wirth and W. E. Walton. 2003. Recent progress on recombinant larvicidal bacteria for mosquito control. 71st Annual Conference of the Mosquito and Vector Control Association of California. Palm Springs, California.

Barboza-Corona, J. E., H.-W. Park and B. A. Federici. 2003. Molecular genetic analysis and enhancement of Cry19A synthesis in Bacillus thuringiensis. 36th Annual Meeting of the Society for Invertebrate Pathology. Burlington, Vermont.

Federici, B. A., M. C. Wirth, J. J. Johnson, H.-W. Park, D. K. Bideshi and W. E. Walton. 2003. Cyt1A synergizes toxicity of Bs Bin by enhancing its insertion through the mosquito midgut microvillar membrane. 36th Annual Meeting of the Society for Invertebrate Pathology. Burlington, Vermont.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2004. The 20-kDa protein of Bacillus thuringiensis subsp. israelensis enhances B. sphaericus 2362 Bin toxin production. 37th Annual Meeting of the Society for Invertebrate Pathology and VIIth International Conference on Bacillus thuringiensis. Helsinki, Finland.

Sakano, Y., H.-W. Park, B. Ge and B. A. Federici. 2004. High level of Cyt1A synthesis in Bacillus thuringiensis subsp. israelensis is due to three promoters and a strong 3’ mRNA stem-loop structure. 37th Annual Meeting of the Society for Invertebrate Pathology and VIIth International Conference on Bacillus thuringiensis. Helsinki, Finland.

Sakano, Y., H.-W. Park and B. A. Federici. 2005. Individual characterization of the three cyt1A promoters of Bacillus thuringiensis subsp. israelensis. 38th Annual Meeting of the Society for Invertebrate Pathology. Anchorage, Alaska.

Park, H.-W., Y. Sakano and B. A. Federici. 2006. A 1.1 kb fragment downstream from the bin operon in Bacillus sphaericus 2362 affects Bin yield and crystal size. 39th Annual Meeting of the Society for Invertebrate Pathology, IXth International Colloquium on Invertebrate Pathology and Microbial Control, and XIIIth International Conference on Bacillus thuringiensis. Wuhan, China.

Sakano, Y., H.-W. Park and B. A. Federici. 2006. Effects of Cyt1A yield reduction on Bacillus sphaericus Bin toxin synthesis in recombinant Bacillus thuringiensis. 39th Annual Meeting of the Society for Invertebrate Pathology, IXth International Colloquium on Invertebrate Pathology and Microbial Control, and XIIIth International Conference on Bacillus thuringiensis. Wuhan, China.

Tang, M., D. K. Bideshi, H.-W. Park and B. A. Federici. 2006. A mini-replicon from pBtoxis of Bacillus thuringiensis subsp. israelensis. 39th Annual Meeting of the Society for Invertebrate Pathology, IXth International Colloquium on Invertebrate Pathology and Microbial Control, and XIIIth International Conference on Bacillus thuringiensis. Wuhan, China.

Park, H.-W., C. M. Mangum, H. Zhong and S. R. Hayes. 2007. Characterization of mosquitocidal and non-mosquitocidal Bacillus sphaericus isolates. 40th Annual Meeting of the Society for Invertebrate Pathology and 1st International Forum on Entomopathogenic Nematodes and Symbiotic Bacteria. Quebec City, Canada.

Tang, M., D. K. Bideshi, H.-W. Park and B. A. Federici. 2007. Interactions between ORF157, ORF156 and the iteron in replication of pBtoxis of Bacillus thuringiensis subsp. israelensis. 40th Annual Meeting of the Society for Invertebrate Pathology and 1st International Forum on Entomopathogenic Nematodes and Symbiotic Bacteria. Quebec City, Canada.

Hayes, S. R., H.-W. Park, G. Stout, M. Latham, G. Day and J. Hunter. 2007. Isolation of bacteria producing novel mosquitocidal toxins. 79th Annual Meeting of the Florida Mosquito Control Association. Jacksonville, Florida.

Nevels, K., H.-W. Park, W. Pathaichindachote, B. Promdonkoy and P. Butko. 2008. Site-directed mutagenesis of the Bt toxin Cyt1A for intramolecular distance determination by FRET. 52nd Annual Meeting of the Biophysical Society and 16th IUPAB International Biophysics Congress. Long Beach, California.

Park, H.-W. and S. R. Hayes. 2008. Characterization of mosquitocidal Bacillus cereus toxic to Ochlerotatus taeniorhynchus and Culex quinquefasciatus. 41st Annual Meeting of the Society for Invertebrate Pathology and 9th International Conference on Bacillus thuringiensis. Coventry, United Kingdom.

Hayes, S. R. and H.-W. Park. 2008. Construction and characterization of recombinant mosquitocidal Cry and Bin toxins to improve efficacy. 1st China – Korea Joint Symposium on Insect Biotechnology. Wuhan, China.

Saleem, F., M. Akhtar, A. R. Shakoori and H.-W. Park. 2008. Characterization of the Cry2Ac7 toxin from a Pakistani isolate of Bacillus thuringiensis (SBS BT-1). 1st China – Korea Joint Symposium on Insect Biotechnology. Wuhan, China.

Saleem, F., H.-W. Park, M. Akhtar and A. R. Shakoori. 2008. Molecular genetic analysis of the cry2Ac11 operon from the HD29 strain of Bacillus thuringiensis subsp. galleriae. 1st China – Korea Joint Symposium on Insect Biotechnology. Wuhan, China.

Hayes, S. R. and H.-W. Park. 2008. Construction and characterization of the chimeric-scanning mutant toxins from Bacillus thuringiensis and Bacillus sphaericus. 2nd Annual Joint Graduate Feeder Conference and Student Research Forum. Florida A & M University, Tallahassee, Florida.

Hayes, S. R. and H.-W. Park. 2008. Chimeric mosquitocidal toxin production for enhancing their efficacy. 80th Annual Meeting of the Florida Mosquito Control Association. Panama City Beach, Florida.

Sun, Y. and H.-W. Park. 2009. Direct analysis of the crystal and spore mixture from six Bacillus thuringiensis strains by a non-electrophoretic in-gel tryptic digestion coupled with mass spectrometry. 1st International Symposium on Insect Biotechnology and 2nd Korea – China Joint Symposium on Insect Biotechnology. Busan, Korea.

Hayes, S. R. and H.-W. Park. 2009. Mosquitocidal activities of Bacillus cereus strains M413 and C32. 1st International Symposium on Insect Biotechnology and 2nd Korea – China Joint Symposium on Insect Biotechnology. Busan, Korea.

Saleem, F., H.-W. Park, M. Akhtar and A. R. Shakoori. 2009. Modification of the nucleotides between RBS and ATG resulted in dramatic increase of Cry2Ac synthesis. 21st IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology. Shanghai, China.

Hayes, S. R., M. Hudon and H.-W. Park. 2009. Identification and characterization of Bacillus species toxic to several mosquito species. 42nd Annual Meeting of the Society for Invertebrate Pathology. Park City, Utah.

Sun, Y. and H.-W. Park. 2009. Proteomic analysis of the crystal and spore mixture from six Bacillus thuringiensis strains to search for novel mosquitocidal proteins. 42nd Annual Meeting of the Society for Invertebrate Pathology. Park City, Utah.

Saleem, F., H.-W. Park, M. Akhtar and A. R. Shakoori. 2009. Overproduction of Cry2Ac by modification of nucleotide sequences between ribosome-binding site and start codon in Bacillus thuringiensis. 42nd Annual Meeting of the Society for Invertebrate Pathology. Park City, Utah.

Wang, Y., P. Kukutla, S. R. Hayes, H.-W. Park and J. Xu. 2009. Anopheles gambiae larval antimicrobial responses upon exposure to a sublethal dose of Bacillus sphaericus. 58th Annual Meeting of the American Society of Tropical Medicine and Hygiene. Washington, D.C.

Hayes, S. R. and H.-W. Park. 2010. Novel mosquitocidal Bacillus species toxic to several mosquito species. 1st Annual CESTA Dean’s Research Forum. Florida A & M University, Tallahassee, Florida.

Bideshi, D. K., G. Waldrop, M. C. Wirth, J. J. Johnson, M. Diaz-Mendoza, H.-W. Park and B. A. Federici. 2010. Cyt1Aa Synergizes the Larvicidal Activity of Cry2Aa against Culex quinquefasciatus through direct intermolecular interaction. 43th Annual Meeting of the Society for Invertebrate Pathology, 10th International Colloquium on Invertebrate Pathology and Microbial Control, and The Final Meeting of COST862: Bacterial Toxins for Insect Control. Trabzon, Turkey.

Saleem, F., H.-W. Park, M. Akhtar and A. R. Shakoori. 2010. Modification of nucleotide sequences between RBS and start codon in Bacillus thuringiensis resulted in increased level of cry2Ac mRNA. 43th Annual Meeting of the Society for Invertebrate Pathology, 10th International Colloquium on Invertebrate Pathology and Microbial Control, and The Final Meeting of COST862: Bacterial Toxins for Insect Control. Trabzon, Turkey.

Zhong, H., H.-W. Park, S. Hayes and T. Ouimet. 2011. Efficacy of novel mosquitocidal Bacillus sp. isolates and their impact on mosquitofish. 77th Annual Meeting of the American Mosquito Control Association. Anaheim, California.

Zhong, H., H.-W. Park, S. Hayes and T. Ouimet. 2011. The efficacy and impact of two novel Bacillus sp. islates. 2nd International Forum for Mosquito Control. Beijing, China.

Barboza-Corona, J. E., H.-W. Park, D. K. Bideshi and B. A. Federici. 2011. The 60-kDa protein encoded by orf2 in the cry19A operon of Bacillus thuringiensis subsp. jegathesan functions as a C-terminus of the 135-kDa Class Cry proteins. 2011 International Congress on Invertebrate Pathology & Microbial Control, OECD Symposium on Disease in Aquatic Crustaceans and 44th Annual Meeting of the Society for Invertebrate Pathology. Halifax, Canada.

Federici, B. A., M. C. Wirth, J. J. Johnson, H.-W. Park, D. K. Bideshi and W. E. Walton. 2011. Cyt1A of Bacillus thuringiensis restores Bacillus sphaericus Bin toxicity via large microvillar lesions, not cation channels, in Bin-resistant larvae of Culex quinquefasciatus. 2011 International Congress on Invertebrate Pathology & Microbial Control, OECD Symposium on Disease in Aquatic Crustaceans and 44th Annual Meeting of the Society for Invertebrate Pathology. Halifax, Canada.

Oliverio, R. M. and H.-W. Park. 2012. Identification and characterization of the novel bipyramidal crystal protein gene in Bacillus thuringiensis subsp. morrisoni PG-14. 37th Annual West Coast Biological Sciences Undergraduate Research Conference. Los Angeles, California.

Park, H.-W., J. A. Devera, B. A. Prins, R. Maynard and D. K. Bideshi. 2012. The dual-activity insecticidal protein, Cry2Aa, does not enhance the mosquitocidal activity of Bacillus thuringiensis subsp. israelensis. 67th Annual Meeting of the American Scientific Affiliation. San Diego, California.

Sun, Y., L. Xia, Q. Zhao, B. A. Federici and H.-W. Park. 2012. Identification of three new Cry proteins synthesized by Bacillus thuringiensis subsp. jegathesan. Insect Pathology Symposium, XXIV International Congress of Entomology. Daegu, Korea.

Tran, J., H.-W. Park and B. A. Federici. 2012. Regulatory genes affect insecticidal protein synthesis in Bacillus thuringiensis. Research in Science and Engineering Summer Research Symposium, University of California, Riverside. Riverside, California.

Pino, B. C., S. Kozervanich-Chong, E. A. Hafkenscheid, R. M. Oliverio, D. K. Bideshi and H.-W. Park. 2013. Cyt1Aa from Bacillus thuringiensis subsp. israelensis enhances mosquitocidal activity of B. thuringiensis subsp. kurstaki HD-1 against Aedes aegypti but not Culex quinquefasciatus. 38th Annual West Coast Biological Sciences Undergraduate Research Conference. San Diego, California.

Oliverio, R. M. and H.-W. Park. 2013. Partial characterization of the 144-kDa protein synthesized by Bacillus thuringiensis subsp. morrisoni PG-14. 5th Annual Research Seminar, California Baptist University, Riverside, California.

Park, H.-W., R. H. Hice and B. A. Federici. 2013. Molecular genetic basis for engineering small to large crystals of Cyt1Aa. 46th Annual Meeting of the Society for Invertebrate Pathology and Conference on Invertebrate Pathology and Microbial Control. Pittsburgh, Pennsylvania.

Sakano, Y., H.-W. Park, B. Ge, D. K. Bideshi and B. A. Federici. 2013. Characterization of a third promoter of the cyt1Aa gene of Bacillus thuringiensis subsp. israelensis. 46th Annual Meeting of the Society for Invertebrate Pathology and Conference on Invertebrate Pathology and Microbial Control. Pittsburgh, Pennsylvania.

Rawley, J. M., A. N. Navarrete, N. R. Bell, E. J. Kettering, D. K. Bideshi and H.-W. Park. 2015. Transformation of Bacillus megaterium for producing insecticidal proteins. 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology. Busan, Korea.

Park, H.-W., R. H. Hice and B. A. Federici. 2015. Effect of single versus multiple promoters and a high plasmid copy number on the synthesis and assembly of Cyt1Aa crystals in Bacillus thuringiensis. International Congress on Invertebrate Pathology and Microbial Control and the 48th Annual Meeting of the Society for Invertebrate Pathology. Vancouver, Canada.

Bideshi, D. K., H.-W. Park, R. H. Hice, M. C. Wirth and B. A. Federici. 2015. Cyt1Aa-BinA chimera highly toxic to anopheline, aedine, and culicine larvae including those tolerant or resistant to Lysinibacillus sphaericus. International Congress on Invertebrate Pathology and Microbial Control and the 48th Annual Meeting of the Society for Invertebrate Pathology. Vancouver, Canada.

Su, T., M. L. Cheng, M. C. Wirth, J. Thieme, H.-W. Park, D. K. Bideshi and B. A. Federici. 2016. Cross resistance to Lysinibacillus sphaericus in Spinosad-resistant Culex quinquefasciatus is overcome by a Cyt1A-BinA recombinant of Bacillus thuringiensis. 82nd Annual Meeting of the American Mosquito Control Association. Savannah, Georgia.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2016. A binB knockout in Lysinibacillus sphaericus demonstrates BinA can form a crystal without BinB in Bacillus thuringiensis. International Congress on Invertebrate Pathology and Microbial Control and the 49th Annual Meeting of the Society for Invertebrate Pathology, Tours, France.

Colletier, J.-P., M. R. Sawaya, M. Gingery, J. A. Rodriguez, D. Cascio, D. K. Bideshi, R. H. Hice, H.-W. Park, B. A. Federici and D. S. Eisenberg. 2016. Lysinibacillus sphaericus Binary toxin structure revelaed in situ by de novo phasing with an X-ray free- electron laser: Insights into the larvicidal biology of BinA and BinB. International Congress on Invertebrate Pathology and Microbial Control and the 49th Annual Meeting of the Society for Invertebrate Pathology, Tours, France.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2017. Cry11B enhances efficacy significantly to Aedes aegypti when added to a Bacillus thuringiensis strain producing the Cyt1A-BinA chimera. Golden Jubilee Conference of the Society for Invertebrate Pathology, San Diego, California.

Bideshi, A. L., H.-W. Park, M. Nelson, J. Monteon, A. Somogyi, D. K. Bideshi and B. A. Federici. 2018. Mosquito larvicidal activity of a recombinant Bacillus thuringiensis harboring a synthetic operon for synthesis of a Cyt1Aa-BinA chimera and Cry11Ba. The 2018 International Congress of Invertebrate Pathology and Microbial Control and the 51st Annual Meeting of the Society for Invertebrate Pathology, Gold Coast, Australia.

Park, H.-W., D. K. Bideshi and B. A. Federici. 2019. Improvement of the recombinant Bacillus thuringiensis strain producing the Cyt1A-BinA chimera by combining with Cry11B. The 2019 Spring Conference of the Korean Society of Applied Entomology. April 24-26, Cheongju, Korea.

SEMINARS, SYMPOSIA, WORKSHOPS AND INVITED PRESENTATIONS:

Park, H.-W. 1997. Genetic engineering of Bacillus thuringiensis for improved Cry3A production. Annual Entomology Graduate Student Seminar Series. University of California, Riverside, California.

Kang, S.-K., Y.-H. Je, B.-R. Jin, H.-W. Park, J.-Y. Roh and J.-H. Chang. 1998. Construction of a novel baculovirus insecticide producing recombinant polyhedra with Bacillus thuringiensis Cry1Ac crystal protein and green fluorescent protein. 31st Annual Meeting of the Society for Invertebrate Pathology, VIIth International Colloquium on Invertebrate Pathology and Microbial Control and IVth International Conference on Bacillus thuringiensis. Sapporo, Japan.

Park, H.-W. 1998. Optimizing endotoxin yields in Bacillus thuringiensis. Annual Entomology Graduate Student Seminar Series. University of California, Riverside, California.

Park, H.-W. 1999. Insect pathology: brief history and recent trends. 1st Regular Meeting of Korean Bioscience Group in University of California, Riverside, California.

Park, H.-W. 1999. Domain I plays a key role in crystallization of Cry3A from Bacillus thuringiensis. Annual Entomology Graduate Student Seminar Series. University of California, Riverside, California.

Park, H.-W. 1999. Molecular genetic analysis and manipulation of truncated Cry protein synthesis and crystallization in Bacillus thuringiensis. Entomology 250 Seminar Series. University of California, Riverside, California.

Park, H.-W. 2000. Molecular genetic analysis and manipulation of truncated Cry protein synthesis and crystallization in Bacillus thuringiensis. The Special Seminar at the Research Center for New Bio-Materials in Agriculture. Seoul National University, Korea.

Park, H.-W. 2000. Manipulation of promoters and mRNA stabilizing sequences to increase insecticidal protein synthesis in Bacillus thuringiensis. Microbiology 250 Seminar Series. University of California, Riverside, California.

Federici, B. A., H.-W. Park and D. K. Bideshi. 2001. Improving the efficacy of Bacillus thuringiensis insecticides: From molecular biology to practical applications. The Centennial Symposium Commemorating Ishiwata’s Discovery of Bacillus thuringiensis. Kurume, Japan.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson and M. C. Wirth. 2001. Synergistic interactions that enhance the pathogenicity of Bacillus thuringiensis. 4th Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Environmental Impact. Canberra, Australia.

Park, H.-W. 2002. Structural role for Domain I helix -7 in Cry3A crystallization in vivo in Bacillus thuringiensis. 5th Regular Meeting of Korean Bioscience Group in University of California, Riverside, California.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson, M. C. Wirth and W. E. Walton. 2002. Genetically engineered bacterial larvicides with markedly improved properties for mosquito control. National Conference of the Mosquito Control Association of Australia. Gold Coast, Australia.

Park, H.-W. 2003. Molecular genetics and genetic engineering of entomopathogenic bacteria. Microbiology 250 Seminar Series. University of California, Riverside, California.

Federici, B. A., H.-W. Park, D. K. Bideshi, M. C. Wirth and J. J. Johnson. 2003. Recombinant bacterial larvicides for mosquito control. The 52nd Annual Meeting of the American Society of Tropical Medicine and Hygiene. Philadelphia, Pennsylvania.

Federici, B. A., H.-W. Park, D. K. Bideshi, Y. Sakano and M. C. Wirth. 2004. From basic research to field application with genetically engineered bacterial insecticides. 37th Annual Meeting of the Society for Invertebrate Pathology and VIIth International Conference on Bacillus thuringiensis. Helsinki, Finland.

Park, H.-W. 2004. Genetic engineering of insecticidal bacteria to control mosquitoes. John A. Mulrennan, Sr., Public Health Entomology Research & Education Center. Florida A & M University. Panama City, Florida.

Park, H.-W. 2004. Genetic engineering of insecticidal bacteria to control mosquitoes. Entomology Program, College of Engineering Sciences, Technology & Agriculture. Florida A & M University. Tallahassee, Florida.

Park, H.-W. 2005. Crystallization and mode of action of Bacillus thuringiensis toxins. John A. Mulrennan, Sr., Public Health Entomology Research & Education Center. Florida A & M University. Panama City, Florida.

Park, H.-W. 2005. Mosquito biological and alternative control. Symposium: Research, extension & education programs conducted for Florida mosquito control in 2005. 77th Annual Meeting of the Florida Mosquito Control Association. Duck Key, Florida.

Park, H.-W. 2006. Current status of pathogenic microorganisms attacking mosquitoes. 10th Annual Southeast Regional Public Health Pest & Vector Management Conference. Panama City Beach, Florida.

Park, H.-W. 2006. Insecticidal bacteria for mosquito control. Department of Life Sciences, The University of the West Indies. St. Augustine, Trinidad.

Federici, B. A., H.-W. Park, D. K. Bideshi, M. C. Wirth, J. J. Johnson, Y. Sakano, M. Tang and W. E. Walton. 2006. Recombinant bacterial larvicides for improving control of nuisance and vector mosquitoes. 87th Annual Meeting of the American Association for the Advancement of Science (Pacific Division). San Diego, California.

Federici, B. A., H.-W. Park, D. K. Bideshi, J. J. Johnson, M. Tang, M. C. Wirth and Y. Sakano. 2006. Improved genetically engineered bacteria for controlling mosquito larvae. 39th Annual Meeting of the Society for Invertebrate Pathology, IXth International Colloquium on Invertebrate Pathology and Microbial Control, and XIIIth International Conference on Bacillus thuringiensis. Wuhan, China.

Park, H.-W. 2006. Development of recombinant bacteria for mosquito control. School of Agricultural Biotechnology, Seoul National University. Seoul, Korea.

Park, H.-W. 2006. USDA grant writing – Improving your grantsmanship. John A. Mulrennan, Sr., Public Health Entomology Research & Education Center, Florida A & M University. Panama City, Florida.

Park, H.-W. and C. M. Mangum. 2006. Microbial insecticide discovery. Symposium: 2006 Synopsis of Florida A & M University – PHEREC mosquito control research & technical support. 78th Annual Meeting of the Florida Mosquito Control Association. Stuart, Florida.

Park, H.-W. 2007. Screening procedure for soil-dwelling mosquitocidal bacteria. 11th Annual Southeast Regional Public Health Pest & Vector Management Conference. Panama City Beach, Florida.

Park, H.-W. 2007. Current status of larvicidal bacteria for mosquito control. 4th Arbovirus Surveillance and Mosquito Control Workshop. St. Augustine, Florida.

Park, H.-W. 2007. Molecular genetic strategies to enhance efficacy of entomopathogenic bacteria. Korea Conference on Innovative Science and Technology 2007. Molecular Host – Parasite Interactions: New Horizon on Interface Biology. Jeju, Korea.

Park, H.-W. 2007. Special ceremony: A new higher toxicity mosquitocidal bacterial isolate, Bacillus sphaericus strain. CESTA 2007 Research Forum, Florida A & M University. Tallahassee, Florida.

Park, H.-W., S. R. Hayes and C. M. Mangum. 2007. Biological control of mosquitoes: Microbiology, molecular genetics and biophysics. CESTA 2007 Research Forum, Florida A & M University. Tallahassee, Florida.

Park, H.-W. and S. R. Hayes. 2007. Research activities on mosquitocidal bacteria. Symposium: FAMU – PHEREC research for Florida mosquito control. 79th Annual Meeting of the Florida Mosquito Control Association. Jacksonville, Florida.

Park, H.-W. 2008. Isolation of mosquitocidal bacteria with improved efficacy from various mosquito habitats in Florida. John A. Mulrennan, Sr., Public Health Entomology Research & Education Center. Florida A & M University. Panama City, Florida.

Park, H.-W. 2008. Isolation of mosquitocidal bacteria with improved efficacy from various mosquito habitats in Florida. Symposium: Pesticides and Environments. 74th Annual Meeting of the American Mosquito Control Association. Sparks, Nevada.

Park, H.-W. 2008. Exploration, development and discovery of insecticidal bacteria. Jamie Whitten Delta States Research Center, Agricultural Research Service, United States Department of Agriculture. Stoneville, Mississippi.

Park, H.-W. 2008. Entomopathogenic bacteria for vector control: Molecular genetic exploration and novel toxin discovery. 1st China – Korea Joint Symposium on Insect Biotechnology. Wuhan, China.

Park, H.-W. 2008. Molecular microbiology. 80th Annual Meeting of the Florida Mosquito Control Association. Panama City Beach, Florida.

Park, H.-W. 2009. Insecticidal bacteria for improving control of vector mosquitoes. Department of Natural and Mathematical Sciences, California Baptist University, Riverside, California.

Park, H.-W. 2009. Defensive strategies of insects. Department of Natural and Mathematical Sciences, California Baptist University, Riverside, California.

Park, H.-W. 2009. Bacterial larvicide research: current status and prospect. 75th Annual Meeting of the American Mosquito Control Association. New Orleans, Louisiana.

Park, H.-W. 2009. Discovery of novel mosquitocidal bacteria from mosquito larval habitats. 1st International Symposium on Insect Biotechnology and 2nd Korea – China Joint Symposium on Insect Biotechnology. Busan, Korea.

Sun, Y., L. Xia, Q. Zhao, B. A. Federici and H.-W. Park. 2012. Identification of three new Cry proteins synthesized by Bacillus thuringiensis subsp. jegathesan. Insect Pathology Symposium, XXIV International Congress of Entomology. Daegu, Korea.

Tran, J., H.-W. Park and B. A. Federici. 2012. Regulatory genes affect insecticidal protein synthesis in Bacillus thuringiensis. Research in Science and Engineering Summer Research Symposium, University of California, Riverside. Riverside, California.

Oliverio, R. M. and H.-W. Park. 2013. Partial characterization of the 144-kDa protein synthesized by Bacillus thuringiensis subsp. morrisoni PG-14. 5th Annual Research Seminar, California Baptist University, Riverside, California.

Park, H.-W., B. C. Pino, S. Kozervanich-Chong, E. A. Hafkenscheid, R. M. Oliverio, B. A, Federici and D. K. Bideshi. 2013. Cyt1Aa from Bacillus thuringiensis subsp. israelensis enhances mosquitocidal activity of B. thuringiensis subsp. kurstaki HD-1 against Aedes aegypti but not Culex quinquefasciatus. 5th Annual Research Seminar, California Baptist University, Riverside, California.

Bell, N. R., E. J. Kettering and H.-W. Park. 2015. Isolation of endospore-forming bacteria from dead mosquito larval sample from Florida. 7th Annual Research Seminar, California Baptist University, Riverside, California.

Rawley, J. M., A. N. Navarrete and H.-W. Park. 2015. Determination of optimal transformation condition for Bacillus megaterium. 7th Annual Research Seminar, California Baptist University, Riverside, California.

Rudd, S. R., D. K. Bideshi, H.-W. Park and B. A. Federici. 2016. Genetic engineering of Bacillus thuringienisis to produce a single chimeric mosquitocidal toxin composed of Cyt1Aa, BinA and Cry11Ba. 8th Annual research Seminar, California Baptist University, Riverside, California.

Searles, S. R., L. A. McCartin, D. K. Bideshi and H.-W. Park. 2016. Lysinibacillus sphaericus: Focus on BinA protein and its ability to crystallization in Bacillus thuringiensis. 8th Annual Research Seminar, California Baptist University, Riverside, California.

Curtis, B. A., E. Luna, D. K. Bideshi and H.-W. Park. 2016. Function of BinB Protein of Lysinibacillus sphaericus 2362. 8th Annual Research Seminar, California Baptist University, Riverside, California.

Park, H.-W., D. K. Bideshi, R. J. Blackwood, Z. T. Saylor, V. Villasenor and B. A. Federici. 2017. Construction of a recombinant Bacillus thuringiensis strain to synthesize mosquitocidal toxins of Cyt1Aa, BinA and Cry11Ba. 9th Annual Research Seminar, California Baptist University, Riverside, California.

Park, H.-W. 2017. Molecular genetic strategies to improve efficacy of entomopathogenic bacteria. Division of Natural and Mathematical Sciences Faculty Colloquium, California Baptist University, Riverside, California.

Bideshi, D. K., H.-W. Park and B. A. Federici. 2018. Biology and pathobiology of Bacillus thuringiensis subsp. israelensis and Lysinibacillus sphaericus, highly efficacious natural mosquito larvicides. Special Seminar, Department of Earth and Biological Sciences, Loma Linda University, Loma Linda, California.

Grossheim, C. M., R. I. Thompson, Y. Bigot, R. H. Hice, H.-W. Park, D. K. Bideshi and B. A. Federici. 2019. Identification of the putative bipyramidal crystal protein gene from Bacillus thuringiensis subsp. morrisoni PG-14. 11th Annual Research Seminar, California Baptist University, Riverside, California.

Fernandez, N., M. Zuniga, K. Hernandez, S. E. Bryant, B. A. Federici, H.-W. Park and D. K. Bideshi. 2019. Genetically engineering microbial factories that produce highly effective combinations of Cry4Aa, Cry4Aa, Cry11Aa, Cyt1Aa, and the novel Cyt1Aa-BinA chimera for applied use in mosquito vector control. 11th Annual Research Seminar, California Baptist University, Riverside, California.

Park, H.-W. 2019. Pathobiology and molecular genetics of highly efficacious mosquitocidal bacteria, Bacillus thuringiensis subsp. israelensis and Lysinibacillus sphaericus. 2019 Spring Annual Meeting of the Korean Society of Sericultural Science, Daejeon, Korea.

Park, H.-W. 2019. Pathobiology and molecular genetics of highly efficacious mosquitocidal bacteria, Bacillus thuringiensis subsp. israelensis and Lysinibacillus sphaericus. Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju, Korea.

Bideshi, D. K., H.-W. Park, M. Diaz-Mendoza, S. R. Rudd, Y. Bigot and B. A. Federici. 2019. The 54-kDa protein of Bacillus thuringiensis subsp. israelensis is required for maturation and stability of its proteinaceous mosquito larvicidal microcompartment. 2019 Fall College of Arts and Sciences Gathering, California Baptist University, Riverside, California.

Federici, B. A., D. K. Bideshi, H.-W. Park, R. H. Hice, W. E. Walton and P. Arensburger. 2020. Recombinant bacteria for mosquito larval control. 3rd Annual Progress & Planning Meeting of the Pacific Southwest Center of Excellence in Vector-Borne Diseases, University of California, Riverside.

Carter, J. D., P. A. Ndowu, D. M. Ruiz, S. D. Borson, D. K. Bideshi, B. A. Federici and H.-W. Park. 2020. Re-engineering Bacillus thuringiensis subsp. israelensis producing Lysinibacillus sphaericus 2362 Bin using homologous recombination. 12th Annual Research Seminar, California Baptist University, Riverside, California.

Jedrzejczak, Y. J., G. E. Espinosa, T. M. Basch, H.-W. Park and D. K. Bideshi. 2020. Determination of the median lethal time (LT50) of a novel Cyt1Aa:BinA protein chimera for applied use in vector control. 12th Annual Research Seminar, California Baptist University, Riverside, California.

Sells, P. J., G. R. Petersen, J. R. Dreschler, S. R. Rudd, B. A. Federici, Y. Bigot, H.-W. Park and D. K. Bideshi. 2021. Expanding the utility of encapsulins in prokaryotes: A novel capsid-based entomopathogenic mirocompartment in Bacillus thuringiensis subsp. israelensis. 13th Annual Research Seminar. March 27, California Baptist University, Riverside, California.

Dennis, K. A., E. E. Ibarra, A. V. Lee, J. C. Kim, R. E. Marks, T. A. Martin, B. A. Federici, D. K. Bideshi and H.-W, Park. 2021. Re-engineering the Bacillus thuringiensis subsp. israelensis strain producing the Lysinibacillus sphaericus Bin toxin by in vivo recombination. 13th Annual Research Seminar. March 27, California Baptist University, Riverside, California.

Dennis, K. A., A. N.-A. Kirk, S. P. Nguyen, D. K. Bideshi and H.-W. Park. 2022. Construction of the plasmid for in vivo homologous recombination in Bacillus thuringiensis. 14th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Quartaro, L. R., A. M. Sarmiento, D. R. Ajtun, G. Y. Baltazar, S. J. Hoover, S. S. Tolentino, D. K. Bideshi and H.-W. Park. 2022. Detection of enterotoxin genes of Bacillus cereus on organic produce. 14th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Mena, O., A. Gutierrez, S. Asariah, E. Espino, H.-W. Park and D. K. Bideshi. 2022. Construction of an artificial operon for synchronous expression of Cyt1Aa and the BinA/B binary mosquito larvicidal toxins. 14th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Sternberg, D., M. Sonerholm, N. Waibel, H.-W. Park and D. K. Bideshi. 2022. A genetic cassette for uniform expression of a Cyt1Aa-BinB chimera and BinA with potential for applied in vector mosquito control. 14th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Park, H.-W. 2022. Molecular genetic and structural analysis of mosquitocidal bacterial toxins. International Symposium on the Celebration of the 60th Anniversary of the Korean Society of Applied Entomology, Gyeongju, Korea.

Rohr, C. J., A. Aguilera, T. U. Nguyen, J. N. Mayorga, S. R. Rudd, D. K. Bideshi and H.-W. Park. 2023. Molecular genetic analysis of the cry2Aa operon in Bacillus thuringiensis subsp. kurstaki HD-1. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Rudd, S. R., L. S. Miranda, H.-W. Park and D. K. Bideshi. 2023. A practical approach for developing a Cyt1Aa-Cry3Aa-based degradable biopesticide that delays or prevents resistance in natural coleopteran pest populations. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Reyes, P. A., W. R. Grayson, S. A. McCullough, E. C.-Y. Chen, L. S. Miranda, S. R. Rudd, H.-W. Park and D. K. Bideshi. 2023. Exploration into the development of efficacious mosquito larvicides: A. Expression Cyt1Aa-Cry11Aa chimera in Bacillus thuringiensis subsp. israelensis. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Calitz, N., E. C. Ewing, J. A. Ferneyhough, I. Rodriguez, L. S. Miranda, S. R. Rudd, H.-W. Park and D. K. Bideshi. 2023. Exploration into the development of efficacious mosquito larvicides: B. Expression Cyt1Aa-Cry11Ba chimera in Bacillus thuringiensis subsp. israelensis. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Mena, O. C., S. J. Asariah, P. E. Hudspeth, S. R. Rudd, H. Curtis, M. A. Alonzo, A. N.-A. Kirk, L. S. Miranda, H.-W. Park and D. K. Bideshi. 2023. Synchronous expression of BinA/BinB and a low level of Cyt1Aa results in a highly efficacious mosquito larvicidal of Bacillus thuringiensis subsp. israelensis. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Guevara, G., R. G. Barnabas, S. R. Rudd, L. S. Miranda, H.-W. Park and D. K. Bideshi. 2023. Engineering more robust strains of Bacillus thuringiensis subsp. israelensis (Bti) that express a highly toxic Cyt1Aa-BinA chimera. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Miranda, L. S., S. R. Rudd, H.-W. Park and D. K. Bideshi. 2023. 3-D Modeling of the 20 kDa chaperone protein of Bacillus thuringiensis subsp. israelensis reveals structural homology with its cytolytic (Cyt) mosquitocidal toxins. 15th Annual NMS Research Symposium, California Baptist University, Riverside, California.

Magnolia Church, Riverside, California
Deacon
Worship Team Member

Editorial Board and Academic Editor, Biological Control
Editorial Board, Biotechnology Letters
Editorial Board and Academic Editor, Toxins
Editor, International Journal of Industrial Entomology
Review Editor, Editorial Board of Insect Molecular Genetics, Frontiers in Insect Science
Member, Nominating Committee, Society for Invertebrate Pathology
Member, Awards & Student Contest Committee, Society for Invertebrate Pathology

And the Lord said, "Simon, Simon! Indeed, Satan has asked for you, that he may sift you as wheat. But I have prayed for you, that your faith should not fail; and when you have returned to Me, strengthen your brethren." Luke 22:31-32 NKJV